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Hamel, L.-P., Sekine, K.-T., Wallon, T., Sugiwaka, Y., Kobayashi, K., Moffett P.
The chloroplastic protein THF1 interacts with the coiled-coil domain of the disease resistance protein N' and regulates light-dependent cell death. Plant Physiology in Press

Yanagisawa, H., Tomita, R., Katsu, K., Uehara, T., Atsumi, G., Tateda, C., Kobayashi, K., Sekine, K.-T.
Combined DECS Analysis and Next-Generation Sequencing Enable Efficient Detection of Novel Plant RNA Viruses. Viruses 8:70. doi:10.3390/v8030070【Open Access】

→ MDPI (

Tasaki, K., Atsumi, G., Nishihara, M., Sekine, K.-T.
Development of a Broad bean wilt virus 2-based expression vector for gentian.
Scientia Horticulture 201:279-286.

→ ScienceDirect (


植物防疫 第70巻 第1号 : 19-22.

→ (社)日本植物防疫協会 (

Imamura, T., Sekine, K.-T., Yamashita, T., Kusano, H., Shimada, H.
Production of recombinant thanatin in watery rice seeds that lack an accumulation of storage starch and proteins.
Journal of Biotechnology (in press)

→ ScienceDirect (

Sekine, K.-T., Shirakawa, A., Yanagisawa, H., Tomita, R., Atsumi, G.
Modification of "DECS analysis", an exhaustive RNA virus diagnostic system, to extend the applicability to various plant species.
PSJ Plant virus disease (in press)


Waliullah, S., Kosaka, N., Yaeno, T., Ali, M.E., Sekine, K.-T., Atsumi, G., Yamaoka, N., Nishiguchi, M., Takahashi, H., Kobayashi, K. Cauliflower mosaic virus Tav protein induces leaf chlorosis in transgenic tobacco through a host response to virulence function of Tav. Journal of General Plant Pathology 81:261-270.

 → Springer Link (

Atsumi, G., Tomita, R. Yamashita, T. and Sekine, K.-T. A novel virus transmitted through pollination causes ring-spot disease on gentian (Gentiana triflora) ovaries. Journal of General Virology 96:431-439.

 → Springer Link (

Atsumi, G.Sekine, K.-T. and Kobayashi, K. New method to isolate total dsRNA. Methods in Molecular Biology 1236:27-37. 

 → Microbiology society (


Waliullah, S., Mochizuki, T., Sekine, K.-T., Atsumi, G., Ali, E., Yaeno, T., Yamaoka, N.,  Nishiguchi, M., and Kobayashi, K. Artificial induction of a plant virus protein in transgenic tobacco provides a synchronous system for analyzing the process of leaf chlorosis. Physiological and Molecular Plant Pathology 88:43-51.

 → ScienceDirect (

Kobayashi, K., Sekine, K.-T., and Nishiguchi, M. Breakdown of plant virus resistance: can we predict and extend durability of virus resistance? Journal of General Plant Pathology 80:327-336. (Review for the 100th anniversary)

 → Springer Link (

Imamura, T., Higuchi, H., Sekine, K.-T., Yamashita, T., and Takahashi, H. High concentrations of sucrose induce overwintering bud formation in gentian plantlets cultured in vitro. Plant Biotechnology 31:97-104.

 → J-STAGE (

Hisa, Y., Suzuki, H., Atsumi, G., Choi, S.H., Nakahara, K.S. and Uyeda, I.  P3N-PIPO of Clover yellow vein virus exacerbates symptoms in pea infected with White clover mosaic virus and is implicated in viral synergism Virology 449:200-2062013

         → ScienceDirect (

(総説) 小林括平・関根健太郎・西口正通 植物ウイルス抵抗性の打破:ウイルス抵抗性の永続性を予測したり延長させたりできるだろうか?日植病報80特集号:165-171

         → 日本植物病理学会報


Atsumi, G., Tomita, R., Kobayashi, K., and Sekine, K.-T. Prevalence and genetic diversity of an unusual virus associated with Kobu-sho disease of gentian in Japan. Journal of General Virology 94:2360-2365.

 → Journal of General Virology (

Atsumi, G., Tomita, R., Kobayashi, K., and Sekine, K.-T. Establishment of an agroinoculation system for broad bean wilt virus 2. Archives of Virology 158:1549-1554. 

   → SpringerLink (

Kobayashi, K., Atsumi, G., Iwadate, Y., Tomita, R., Chiba, K., Akasaka, S., Nishihara, M., Takahashi, H., Yamaoka, N., Nishiguchi, M., and Sekine, K.-T. Gentian Kobu-sho-associated virus: a tentative, novel double-stranded RNA virus that is relevant to gentian Kobu-sho syndrome. Journal of General Plant Pathology 79:56-63.

   → SpringerLink (

寄稿関根健太郎・小林括平 植物ウイルス抵抗性タンパク質による病原体認識の特異性決定機構BIOSCIENCE & INDUSTRY 71:23-26

 → 一般財団法人 バイオインダストリー協会 機関誌(

Choi, S.H., Hagiwara-Komoda, Y., Nakahara, K.S., Atsumi, G., Shimada, R., Hisa, Y., Naito, S., and Uyeda, I. Quantitative and Qualitative Involvement of P3N-PIPO in Overcoming Recessive Resistance against Clover Yellow Vein Virus in Pea Carrying the cyv1 Gene.  Journal of Virology 87:7326–7337

   →   ASM (


Sekine, K.-T. , Tomita, R., Takeuchi, S., Atsumi, G., Saitoh, H., Mizumoto, H., Kiba, A., Yamaoka, N., Nishiguchi, M., Hikichi, Y., and Kobayashi, K. Functional differentiation in the LRR domains of closely related plant virus resistance proteins that recognize common Avr proteins. Molecular Plant-Microbe Interactions 25:1219-1229.

 → APS Journals (

Kobayashi, K., Atsumi, G., Yamaoka, N., and Sekine, K.-T. Sequencing-based virus hunting and virus detection. Japan Agricultural Research Quarterly 46:123-128.


Mizumoto, H., Nakamura, I., Shimomoto, Y., Sawada, H., Tomita, R., Sekine, K.-T., Kiba, A., Nishiguchi, M., Kobayashi, K., and Hikichi, Y. Amino acids in Tobamovirus coat protein controlling pepper L1a gene-mediated resistance. Molecular Plant Pathology 13:915-922

 → WILEY (

Takahashi, H., Kai, A, Yamashita, M., Ando, S., Sekine, K.-T., Kanayama, Y., and Tomita, H. Cyclic nucleotide-gated ion channel-mediated cell death may not be critical for R gene-conferred resistance to Cucumber mosaic virus in Arabidopsis thaliana. Physiological and Molecular Plant Pathology 79:40-48

 → (

Atsumi, G., Nakahara, K.S., Wada, T.S., Choi, S.H., Masuta, C., and Uyeda, I. Heterologous expression of viral suppressors of RNA silencing complements virulence of the HC-Pro mutant of clover yellow vein virus in pea. Archives of Virology 157: 1019-1028

 → SpringerLink (


Tomita, R., Sekine, K.-T., Mizumoto, H., Sakamoto, M., Murai, J., Kiba, A., Hikichi, Y., Suzuki, K., and Kobayashi, K. Genetic basis for the hierarchical interaction between tobamoviruses and allelic L resistance genes from different pepper species. Molecular Plant-Microbe Interactions 24: 108-117.

 → MPMI (

Hada, H. and Sekine, K.-T. A diagnostic multiplex polymerase chain reaction method to identify Japanese internal apple-feeding Lepidopteran pests: Grapholita molesta, Grapholita dimorpha (Lepidoptera: Tortricidae), and Carposina sasakii (Lepidoptera: Carpoinidae). Applied Entomology and Zoology 46:287-291.

 → SpringerLink

小林括平・山岡直人・西口正通・関根健太郎 トウガラシ属植物のトバモウイルス抵抗性遺伝子L の単離と機能解析.植物防疫 65:239-243

Chanda, B., Xia, Y., Mandal, M.K., Yu, K., Sekine, K.-T., Gao, Q.M., Selote, D., Hu, Y., Stromberg, A., Navarre, D., Kachroo, A., and Kachroo, P. (2011) Glycerol-3-phosphate is a critical mobile inducer of systemic immunity in plants. Nature Genetics 43:421-427

Mandal, M.K., Chanda, B., Xia, Y., Yu, K., Sekine, K.-T., Gao, Q.M., Selote, D., Kachroo, A., and Kachroo, P.  Glycerol-3-phosphate and systemic immunity. Plant Signaling and behavior 6: 1871-1874 

Nakahara, K.S., Kitazawa, H., Atsumi, G., Choi, S.H., Suzuki, Y. and Uyeda, I. Screening and analysis of genes expressed upon infection of broad bean with Clover yellow vein virus causing lethal necrosis. Virology journal 8: 355-360.